Uji Kepekaan Sel Biofilm Isolat Klinik Staphylococcus aureus Penyebab OMSK di RSUP dr. Sardjito Terhadap Antibiotik Vancomycin Secara In Vitro
Background: Chronic Suppurative Otitis Media (CSOM) is a chronic middle ear infection that usually happened in childhood and can cause hearing problems. Staphylococcus aureus as one of the causative agents is highly potential in developing biofilm that further complicates CSOM treatment. Currently t...
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| Main Authors: | , |
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| 格式: | Theses and Dissertations NonPeerReviewed |
| 出版: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| 主題: | |
| 在線閱讀: | https://repository.ugm.ac.id/128307/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68647 |
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| 總結: | Background: Chronic Suppurative Otitis Media (CSOM) is
a chronic middle ear infection that usually happened in
childhood and can cause hearing problems.
Staphylococcus aureus as one of the causative agents is
highly potential in developing biofilm that further
complicates CSOM treatment. Currently there is a
reduced susceptibility of S. aureus to vancomycin,
which is commonly used to treat planktonic
staphylococcal infection, while a higher dose is needed
to treat biofilm.
Objectives: To evaluate the susceptibility of S. aureus
biofilm to vancomycin in vitro, particularly Minimum
Biofilm Reduction Concentration 50 (MBRC50).
Methods: This study used a descriptive observational
method. Samples were obtained from middle ear secretion
of patients with CSOM in ENT polyclinic, Dr. Sardjito
Hospital during October-December 2012. S. aureus was
grown in vitro as biofilm and exposed to different
concentrations of vancomycin. MTT assay was used to
determine MBRC50.
Results: In the concentration of 32 μg/ml, 50%
reduction of S. aureus biofilm growth was observed.
Conclusion: Vancomycin was able to achieve MBRC50 for
S. aureus biofilm in the concentration of 32 μg/ml. |
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