PREVALENSI INFEKSI HEPATITIS B TERSAMAR DENGAN MUTASI DI REGIO PRECORE DAN ANALISIS SUBGENOTIPE PADA PENDONOR DARAH DI UNIT PELAYANAN TRANSFUSI DARAH RUMAH SAKIT UMUM PUSAT DR. SARDJITO
Background: HBV infection is a serious problem with 2 billion infected worldwide and Indonesia has moderate to high endemicity. HBV are divided into 8 genotypes (A-H). Genotype B is the most commonly found genotype in Indonesia. HBV is the most commonly transmitted virus through transfusion which oc...
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| Main Authors: | , |
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| 格式: | Theses and Dissertations NonPeerReviewed |
| 出版: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| 主題: | |
| 在線閱讀: | https://repository.ugm.ac.id/127921/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68235 |
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| 機構: | Universitas Gadjah Mada |
| 總結: | Background: HBV infection is a serious problem with 2
billion infected worldwide and Indonesia has moderate
to high endemicity. HBV are divided into 8 genotypes
(A-H). Genotype B is the most commonly found genotype
in Indonesia. HBV is the most commonly transmitted
virus through transfusion which occurrence is possibly
caused by OBI. OBI is associated with escape mutation.
Precore mutation is one of the types of HBV escape
mutant. Therefore, the researcher would like to find
out the prevalence of OBI patients with precore
mutation and the genotype/subgenotypes that are found
in voluntary blood donors in RSUP DR. Sardjito.
Objectives: To give information about the subgenotype,
the prevalence of OBI patients with precore mutations,
and nucleotide location of precore mutations in
voluntary blood donors with OBI.
Methods: This research is an observational descriptive
study. Samples used are blood that has passed the Blood
Transfusion Unit screening (including HBsAg screening)
where the serum will be separated and will go through
serological test including HBsAg, anti-HBs, and anti-
HBc. All samples will go through PCR and
electrophoresis where HBV positive control from Anatomy
Pathology Laboratorium of Medical Faculty UGM will be
used for comparison. The samples that are identified
positive HBV DNA will go through sequencing to see the
mutation location and afterwards the subgenotype of the
samples will be analised using PubMed Blast.
Results: 32 out of 150 (21,3%) were anti-HBs positive.
50 out of 150 (33,3%) were anti-HBc positive. 18 out of
150 (12%) were identified OBI because the samples
contained HBV DNA. Mutation in the precore region was
observed in 4 out of 18 samples where G1896A mutation
was observed in 2 samples and G1896A/G1899A in the
other 2 samples. Subgenotype B3 was detected in all
samples.
Conclusion: Precore mutation was found in 4 out of 18
samples of OBI where G1896A mutation was observed in 2
samples and G1896A/G1899A was observed in the other 2
samples and all of which has subgenotype B3. |
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