PREVALENSI INFEKSI HEPATITIS B TERSAMAR DENGAN MUTASI DI REGIO PRECORE DAN ANALISIS SUBGENOTIPE PADA PENDONOR DARAH DI UNIT PELAYANAN TRANSFUSI DARAH RUMAH SAKIT UMUM PUSAT DR. SARDJITO

• Main Authors: , NABILA RAHMANIA, , dr. Didik Setyo Heriyanto, Sp.PA, Ph.D
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/127921/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68235

Background: HBV infection is a serious problem with 2 billion infected worldwide and Indonesia has moderate to high endemicity. HBV are divided into 8 genotypes (A-H). Genotype B is the most commonly found genotype in Indonesia. HBV is the most commonly transmitted virus through transfusion which occurrence is possibly caused by OBI. OBI is associated with escape mutation. Precore mutation is one of the types of HBV escape mutant. Therefore, the researcher would like to find out the prevalence of OBI patients with precore mutation and the genotype/subgenotypes that are found in voluntary blood donors in RSUP DR. Sardjito. Objectives: To give information about the subgenotype, the prevalence of OBI patients with precore mutations, and nucleotide location of precore mutations in voluntary blood donors with OBI. Methods: This research is an observational descriptive study. Samples used are blood that has passed the Blood Transfusion Unit screening (including HBsAg screening) where the serum will be separated and will go through serological test including HBsAg, anti-HBs, and anti- HBc. All samples will go through PCR and electrophoresis where HBV positive control from Anatomy Pathology Laboratorium of Medical Faculty UGM will be used for comparison. The samples that are identified positive HBV DNA will go through sequencing to see the mutation location and afterwards the subgenotype of the samples will be analised using PubMed Blast. Results: 32 out of 150 (21,3%) were anti-HBs positive. 50 out of 150 (33,3%) were anti-HBc positive. 18 out of 150 (12%) were identified OBI because the samples contained HBV DNA. Mutation in the precore region was observed in 4 out of 18 samples where G1896A mutation was observed in 2 samples and G1896A/G1899A in the other 2 samples. Subgenotype B3 was detected in all samples. Conclusion: Precore mutation was found in 4 out of 18 samples of OBI where G1896A mutation was observed in 2 samples and G1896A/G1899A was observed in the other 2 samples and all of which has subgenotype B3.