SUPLEMENTASI NANOPARTIKEL DAUN KELOR (Moringa Oleifera Lam.) TERHADAP KADAR MDA (MALONDIALDEHID) DAN EKSPRESI CASPASE-3 DALAM TAHAP MATURASI OOSIT SECARA IN VITRO
Successful in vitro fertilization (IVF) needs high quality mature oocytes for optimum results during in vitro maturation (IVM). If the temperature in the incubator is not optimal, the condition of culture, protein supplementation, pH also becomes not optimal, and leads to an increase in the produ...
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| 主要作者: | |
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| 格式: | Theses and Dissertations NonPeerReviewed |
| 語言: | Indonesian Indonesian Indonesian Indonesian |
| 出版: |
2019
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| 主題: | |
| 在線閱讀: | http://repository.unair.ac.id/93199/1/TKR.%2027-19%20Kus%20s%20ABSTRAK.pdf http://repository.unair.ac.id/93199/2/TKR.%2027-19%20Kus%20s%20DAFTAR%20ISI.pdf http://repository.unair.ac.id/93199/3/TKR.%2027-19%20Kus%20s%20DAFTAR%20PUSTAKA.pdf http://repository.unair.ac.id/93199/4/TKR.%2027-19%20Kus%20s%20BR.pdf http://repository.unair.ac.id/93199/ http://lib.unair.ac.id |
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| 總結: | Successful in vitro fertilization (IVF) needs high quality mature oocytes for
optimum results during in vitro maturation (IVM). If the temperature in the
incubator is not optimal, the condition of culture, protein supplementation, pH also
becomes not optimal, and leads to an increase in the production of reactive oxygen
species (ROS). Increased ROS due to high temperatures initiates a lipid
peroxidation and the end products are malondialdehyde (MDA) levels and leading
activation caspase-3 expression. More attention has been given to the antioxidant
supplementation such as Moringa oleifera (MO) leaf with nanoparticle during IVM.
With small size, nanoparticles shows closer to the surface of damaged cell due to
the production of ROS. This study aimed to analyzed the effect of moringa oleifera
leaf nanoparticle supplementation to malondialdehyde levels and caspase-3
expression during in vitro maturation. This study was true experimental with posttest
only control group design, used goat oocyte, which each the treatment group
(n=31) was supplementation dosage 1.0 and 2.0 μM with MO leaf nanoparticle.
Maturation process was done in CO2 incubator 5% at different temperature, 3
groups 38.5ºC and 3 groups 41ºC for ±20-22 hours. The results of the performed
statistical test MDA levels (38.5°C p=0.276; 41°C p= 0.001), and caspase-3
expression (38.5°C p=0.046; 41°C p= 0.028) significantly different (p<0.05) with
MO leaf nanoparticle supplementation during IVM. Conclusion: supplementation
of MO leaf nanoparticle has effect to reduce MDA levels and caspase-3 expression
during IVM. |
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