การสังเคราะห์ควอนตัมดอทติดประจุบวกที่ละลายน้ำได้เพื่อการประยุกต์ใช้เป็นเซ็นเซอร์ทางการแพทย์
Quantum dots are nanocrystals of semiconductors possessing unique fluorescence properties that depend on the size of the particles. The ease of synthesis of quantum dots with controllable size and emission wavelength together with the excellent photostability and the ability to functionalize the sur...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | |
| التنسيق: | Senior Project |
| اللغة: | Thai |
| منشور في: |
จุฬาลงกรณ์มหาวิทยาลัย
2015
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://digiverse.chula.ac.th/Info/item/dc:9932 |
| الوسوم: |
إضافة وسم
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| الملخص: | Quantum dots are nanocrystals of semiconductors possessing unique fluorescence properties that depend on the size of the particles. The ease of synthesis of quantum dots with controllable size and emission wavelength together with the excellent photostability and the ability to functionalize the surface of quantum dots make them an attractive fluorophore to be used in various applications such as labeling of biological molecules and biosensors. In this work, we synthesized water soluble CdTe quantum dots carrying charged ligands for DNA detection applications. First, CdTe quantum dots were synthesized from CdCl₂, Na₂TeO₃, NaBH₄ and N₂H₄·H₂O and cysteamine, L-cysteine, thiosalicylic acid, L-histidine and 2-mercaptoethanol as ligands. It was accidentally discovered that exposure to UV radiation caused a cysteamine-capped CdTe quantum dot changed its color from green to red upon UV irradiation at 365 nm for 10 minutes, which was confirmed by fluorescence spectroscopy. This discovery provided a new way for controlling the emission color of the quantum dots by UV irradiation. In addition, some preliminary experiments were carried out to demonstrate the use of quantum dots as a stain for detection of DNA immobilized on cellulose paper and the use of negatively-charged quantum dots with 3-mercaptopropionic acid ligand in combination with Nile red labeled pyrrolidinyl peptide nucleic acid (acpcPNA) for DNA sequence determination in a solution. While the former was not yet successful due to the limited stability of the synthesized quantum dots, the latter experiment clearly demonstrated the applicability of quantum dots for DNA sensing in solution. |
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