ฟลูออเรสเซนต์ที่มีฐานเป็นอิมิดาโซเลียมและกรดโบโรนิกสำหรับตรวจวัดสารประกอบเอมีนทางชีวภาพ
Histamine blue was synthesized via mesoionic reaction from isoquinoline, TFAA, and methyl isocyanoacetate with 23% yield. The binding affinities with biogenic amines including histamine and histidine, were investigated by fluorescence spectrophotometry. Histamine blue shows the fluorescence signal a...
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| Format: | Senior Project |
| Language: | Thai |
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จุฬาลงกรณ์มหาวิทยาลัย
2013
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| Online Access: | https://digiverse.chula.ac.th/Info/item/dc:9798 |
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| Institution: | Chulalongkorn University |
| Language: | Thai |
| Summary: | Histamine blue was synthesized via mesoionic reaction from isoquinoline, TFAA, and methyl isocyanoacetate with 23% yield. The binding affinities with biogenic amines including histamine and histidine, were investigated by fluorescence spectrophotometry. Histamine blue shows the fluorescence signal at 400 nm. Upon the interaction with histamine and histidine, Histamine blue adduct performed the bathochromic shift with the emission band at 410 nm. A similar fluorescence change cannot differentiate Hist and His regarding to non-specificity of histamine blue for sensing purpose. Therefore, NapIm2 and NapIm3 were designed as sensors to discriminate Histamine and Histidine. The carboxylic acid in histidine is expected to preferentially bind with NapIm3 resulting in fluorescence-on aspect at 525 nm with excitation wavelength at 340 nm while histamine cannot perform this phenomena. Notably, histamine blue can discriminate histamine and histidine from other biogenic amines and NapIm3 shows fluorescence-on with only histidine. Therefore, the multi-sensors offer a promising discrimination between histamine and histidine. |
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